Journal: Journal of Neuroinflammation

Article Title: Age-dependent involvement of gut mast cells and histamine in post-stroke inflammation

doi: 10.1186/s12974-020-01833-1

Figure Lengend Snippet: Increased levels of histamine receptor 2 (HR2) measured in the intestinal samples of young and aged mice for 6-h, 24-h, and 7-days post-stroke compared to the age matched controls. a mRNA expression levels of HR2 quantified by qPCR in young and aged mice at 6 and 24 h post-stroke. b (i) Protein levels of HR2 quantified by IHC followed by image J quantification in aged mice at 7 days post-stroke and c mRNA levels of HR2 quantified by RNA in situ hybridization and analyzed by image-J in the intestine of Ag mice at 7 days post-stroke. n = 3 (sham) and 5 (stroke) per group ( a ) and 5 (sham) and 5 (stroke) per group ( b and c ). Data are expressed as mean ± SEM ( a ), ( b ), and ( c ), as well as individual values. * P < 0.05, ** P < 0.01, *** P < 0.001. P values were calculated using one-way analysis of variance with Tukey multiple comparisons correction ( a ) or with two-tailed unpaired Student’s t test ( b and c ). b Brown-HR2 protein; blue-Nuclei ( b ) purple dots (red arrows)-mRNA HR2

Article Snippet: Formalin fixed, paraffin-embedded intestinal (cecal, ileum) tissue sections (4 μm) were incubated overnight at 4 °C with a primary antibody targeting the mouse antigens (1) histamine receptor 2 (AHR-002, Alomone labs, Israel), (2) Tryptase (ab2378, Abcam, USA), after antigen retrieval according to the manufacturer’s instructions.

Techniques: Expressing, RNA In Situ Hybridization, Two Tailed Test